A Simple and Fast Method for Detecting Glucose in Wines Using a Redox Polymer-Based Amperometric Biosensor
نویسندگان
چکیده
In our quest for a simple and fast method to detect glucose in wines, a redox polymer (RP)-based amperometric biosensor was developed. The biosensor consists of glucose oxidase and horseradish peroxidase (as recognition tandem), an Os-based RP (as electron transfer mediator), poly(ethylene glycol) diglycidyl ether (as cross-linking agent), and a graphite electrode (as transducer). Each building block of the biosensor was carefully investigated. First, the electrochemical behaviour of the surface immobilized Osbased RP was investigated. Peroxidaseand RP-containing layers were subsequently studied using normal pulse voltammetry (NPV). Finally, glucose oxidase was also added to the sensing layer, and the obtained sensor was calibrated and used to detect glucose in wine. The obtained values agree well with concentrations reported by the producer.
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